Please follow the steps provided below to troubleshoot the 16-bit sub-system error:
1. Click Start, click Run, type c:\windows\repair, and then click OK.
2. Right-click autoexec.nt, and then click Copy.
3. Click Start, click Run, type c:\windows\system32, and then click OK.
4. Right-click anywhere in that folder, and then click Paste.
5. Right-click the Autoexect.nt file that you just copied, and then click Properties.
6. Click to select Read-Only, and then click OK.
7. Repeat steps 1 through 6 to copy the Config.nt file.
8. Restart your computer and trying running the application.
For additional information, I suggest you read the following article from Microsoft:
*support.microsoft.com/kb/324767
Ajay M (MCP)
Bangalore
Mob: +91-9886779379
web: *www.azoo.co.nr